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| Title | Cell Biology Laboratory Speciality: Vascular Biology |
|---|---|
| Extension no. | 32448, 32447 |
| Location | BS207 |
| Participating professor | Wei-Ting Chao |
| Facility | |
| Research direction | It has been recognized that the high-density lipoprotein (HDL) plays an important role in the prevention of atherogenic plaque formation. However, there is considerable debate on the mechanisms involved in HDL-mediated cholesterol efflux from cells. The non-coated vesicular structure "caveolae" which have been implicated in cellular transport were recognized as flask-shaped indentations of the cell membrane a half century ago. Biochemical analysis indicates that this structure plays an important role in the regulation of cellular cholesterol homeostasis. However, the interaction among caveolin-1, a main structural component of caveolae,ABCA1,and HDL is still unknown. Since this information is important to further elucidate the underlying mechanisms of HDL-mediated cholesterol efflux from cell, our work focuses on the location and distribution of caveolin-1 and ABCA1 in the cholesterol-loaded cells after HDL exposure. Other projects undergoing in our laboratory include: 1. reconstruction of 3D structure of caveolae by confocal and electron microscopy, 2. distribution and expression of ATP binding cassete membrane transporter ( ABCA1) and caveolin-1 in cholesterol-loaded endothelial cells after exposure to HDL, 3. cholesterol efflux in caveolin-1- and ABCA1-overexpressed and down-regulated endothelial cells 4. redistribution of caveolin-1 in the down-reulated ABCA1 endothelial cells and the underlying mechanisms on the cellular cholesterol efflux |
| Students | Postdoctoral fellow: Dr. Chan-Yen Kuo PH.D. students: HC Hou(MD), MJ Hong(MD), SH Tsai MS student BH Lee Junior students: HC Ling, CH Hong, HC Chang |
| Image | |
| Reference link | http://www.thu.edu.tw/web/news/news_detail.php?cid=8&id=962 |
